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Method to include insertions in compact alignment from BioMap object?

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Turner
Turner 2013 年 5 月 25 日
閉鎖済み: MATLAB Answer Bot 2021 年 8 月 20 日
Hi all,
I'm working with a BioMap object containing 50bp sequencing reads from a BAM file. My goal is to retrieve a full genome sequence from the BioMap. So far, I know how to use getCompactAlignment to get a memory-friendly version of an alignment that can be converted into a consensus sequence. I've mastered how to handle deletions, but getCompactAlignment does not account for insertions, and actually removes them from the alignment. Is there another method to generate a compact alignment or direct consensus sequence from a BioMap that includes insertions?

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