How can I create a 3D Tiff image (2D stack) from a 3D matrix?

I have to save a 400x400x400 matrix (bw) in a stack of 2D tiff images.

I tried to us "imwrite" but it gives me this error:

Error using writetif (line 40)

Writing TIFFs with 400 components is not supported with IMWRITE. Use Tiff instead. Type "help Tiff" for more information.

Error in imwrite (line 546)

feval(fmt_s.write, data, map, filename, paramPairs{:});

But typing "help Tiff" I didn't find any solution.

Answer 1

[Tim]

Loop over your volume using imwrite(image_page_1, filename) for n == 1 and imwrite(image_page_nLargerThan1, filename, 'WriteMode', 'append') for n > 1. Here's some code I use to do this on a regular basis:

% Your example volume
tst = randn(400, 400, 400);

% Normalize for RGB colormap mapping:
scaled_tst = tst./max(tst, [], 'all');

% Colormap
cmp = parula;

% Write flag: run once with iwrite = false if you want to see what you are writing first
iwrite = true;

% File name:
fname = 'Test.tif';

for n = 1:size(tst, 3)
    
    % Make an RGB image:
    i_img = ind2rgb(round(scaled_tst(:, :, n)*256), cmp);
    
    % Check what you are writing
    image(i_img);
    drawnow;
    
    % Generate your tiff stack:
    if iwrite
        if n == 1
            % First slice:
            imwrite(i_img,fname)
        else
            % Subsequent slices:
            imwrite(i_img,fname,'WriteMode','append');
        end 
    end
    
    disp(n)
end