i need to calculate certain parameters for chromosome in the image. how can i select region of chromosome alone from the background to calculate parameters.

[manoj m]

check image in the link : http://imageshack.us/photo/my-images/138/chromosomeimage.png/

Answer 1

[Image Analyst]

Threshold:

binaryImage = grayImage > thresholdValue;
measurements = regionprops(binaryImage, 'all');

[manoj m]

sir,i want to calculate statistical parameters like variance,entropy,mean to the chromosome region in the image. these parameters are not available in regionprops. Is there any other way by which i can get the pixel values in the chromosome region and calculate those parameters.

[Image Analyst]

regionprops() will return the pixel values, as will

pixelValues = grayImage(binaryImage);