Matlab Bioinformatics toolbox error "Reference to non-existant field 'Sequence'"

I am using Matlab Bioinformatics toolbox and have encountered an error regarding the field 'Sequence' several times.

When I try to load a sequence into the Biological Sequence Viewer as per the online instructions (https://www.mathworks.com/help/bioinfo/ug/importing-viewing-and-exploring-a-nucleotide-sequence-using-a-graphical-interface.html), I get a pop-up window with the error, "Reference to a non-existant field 'Sequence' "

The sequence accession number I'm using is: NC_000964

I've tried different combinations with and with out the underscore and ".3" at the end (NC_000964.3) but without success.

I get a similar error when trying to analyze synonymous and nonsynonymous substitution rates as per the instructions (https://www.mathworks.com/help/bioinfo/examples/analyzing-synonymous-and-nonsynonymous-substitution-rates.html).

Here I get the error: "Reference to non-existent field 'Sequence'."

Would anyone possibly have suggestions for me?

Thanks!

Answer 1

[Sindhu Karri]

Hii,

The record (NC_000964)which you are trying to access doesn't contain sequence information,because of which you are facing the issue.

You can access the record in the following way:

hiv1 = getgenbank('NC_000964', 'FILEFORMAT', 'FASTA');
seqviewer(hiv1)