If you're using GUIDE, then all callbacks (what gets executed when you interact with some control on the GUI) will automatically have access to handles. If you don't have it, then you must have deleted it from the input argument list, or called "clear" in the callback function itself.
OTHER (non-callback) functions will not automatically have access to the handles structure variable. To get them to have access, you have to pass in handles to them when you call the functions. Of course the calling function must also have access to handles, such as the caller being a callback function or a custom user-written function that has had handles passed into it. For example, you have a push button callback function call a function Fungsiocr() which is your last chunk of code:
[colorImage, handles] = Fungsiocr(handles, datacitrargb, dataaxes)
Make sure you change the function definition of Fungsiocr() to include handles as the first input argument.
You don't need to pass handles back out if you call guidata() inside Fungsiocr() since that will update handles so that other, later functions will see the changes you made. Otherwise, if you don't call guidata() in Fungsiocr(), then pass handles back out and some other function will eventually call it.
