Add "drawnow" at the beginning of the function like this:
function startupFcn(app)
drawnow
fig_position = app.UIFigure.Position;
fig_width = fig_position(3);
fig_height = fig_position(4);
app.GridLayout.ColumnWidth = {0.54*fig_width, 0.46*fig_width};
app.GridLayout.RowHeight = fig_height;
end