measurePSF
This function measures the full-width-half-max of a microscope point spread function (PSF) along both x and y at the z-plane with the brightest intensity, and also along z/x and z/y. The user simply needs to provide a z-stack containing a PSF (e.g. by imaging a sub-micron fluorescent bead) and also the number of microns per pixel along x/y and z. If using ScanImage (2-photon scanning software) the number of microns per pixel are automatically extracted from the TIFF header.
This submission also contains code and information to measure the number of microns per pixel using an EM grid. If using ScanImage, there is a button in the GUI to apply the measured calibration to ScanImage.
引用
Rob Campbell (2024). measurePSF (https://github.com/SWC-Advanced-Microscopy/measurePSF), GitHub. 取得済み .
MATLAB リリースの互換性
プラットフォームの互換性
Windows macOS Linuxカテゴリ
タグ
Community Treasure Hunt
Find the treasures in MATLAB Central and discover how the community can help you!
Start Hunting!code
code/+mpsf
code/+mpsf/+plot
code/+mpsf/+record
code/+mpsf/+record/private
code/+mpsf/+report
code/+mpsf/+settings
code/+mpsf/+tools
code/+mpsf/+yaml
code/+mpsf/+yaml/Tests
code/+mpsf/+yaml/extras
code/+sibridge
code/@Grid2MicsPerPixel
code/@measurePSF
GitHub の既定のブランチを使用するバージョンはダウンロードできません
バージョン | 公開済み | リリース ノート | |
---|---|---|---|
2.0.0.0 | Update what the submission does. |
|
|
1.0.0.0 |
|