How can I count relative proportion of Green/Red pixels in a merged green and red channel fluorescence image?

Hi there,

I am new with Matlab. I have merged Green/Red fluorescence images of my bacterial colony with strains that express GFP et mCherry (see image here) and I want to count the relative proportion of Green and Red pixels in my colony. Could anyone help me?

Thanks in advance!

Regards,

Fabio Palmieri

[Stephan]

Hi. The link is dead. Attach pictures in your question.

Best regards

Stephan